genotoxicidade

Determinação da frequência de micronúcleos em células esfoliativas da mucosa oral em indivíduos fumantes e etilistas

  • Autores:
  • Luan Aércio Melo Maciel
  • Deusiane Ribeiro da Silva
  • Suany Vasconcelos da Silva
  • Talita Castro
  • Luana Santos da Costa
  • Thais Sena Trolly
  • Isabela Natilde Costa Goes
  • Samaroni Brelaz Feitosa
  • Albino Luciano Portela de Sousa
  • Palavras-chave:
  • álcool
  • câncer
  • fumo
  • genotoxicidade
  • micronúcleo
Resumo: Smoking has been described as the main risk factor in the development of malignant and premalignant lesions in the oral mucosa, and alcohol consumption, on the other hand, is cited as a strong potential agent in triggering cancerous lesions. The study aimed to evaluate the frequency of micronuclei in exfoliative cells of the oral mucosa of smokers and alcoholics, comparing them with non-exposed individuals. Forty volunteers were selected and distributed in 4 groups containing individuals exposed to smoking and alcohol and not exposed. To determine the micronucleus frequency, the micronucleus assay technique was used in oral mucosal cells. The cells examined were collected using cytobrush brush and stained with Feulgen/Fast-green techniques. In the present study, the frequency of micronuclei in the groups exposed to tobacco and alcohol genotoxicity was significantly higher (P < 0.01) when compared to the control group. The association between alcohol and tobacco increased the appearance of micronucleus in the continuous users of these two substances (P < 0.05). Smokers and consumers of alcoholic beverages present higher amounts of cytotoxic damage to oral mucosa cells caused by the action of carcinogens present in cigarette smoke and alcohol. Keywords: micronucleus, genotoxicity, cancer, smoking, alcohol. Expandir Resumo Acessar Texto Completo